537例结肠腺癌患者KRAS基因检测结果分析

doi:10.3969/j.issn.1000-8535.2018.04.002

广州医药 ›› 2018, Vol. 49 ›› Issue (4): 7-9.DOI: 10.3969/j.issn.1000-8535.2018.04.002

537例结肠腺癌患者KRAS基因检测结果分析

李秀博¹, 杜洪¹, 王小拍¹, 李雯¹, 沈春辉²

1 广州市第一人民医院病理科分子实验室(广州 510180);
2 广州中医药大学第一附属医院心胸外科(广州 510405)

收稿日期:2018-03-06 出版日期:2018-07-20 发布日期:2021-11-29
通讯作者: 沈春辉,E-mail:1023149582@qq.com

Retrospectively analysis of 537 colonic adenocarcinoma patients with KRAS gene detect

LI Xiubo¹, DU Hong¹, WANG Xiaopai¹, LI Wen¹, SHEN Chunhui²

1 Molecular Laboratory of Pathology, Guangzhou First People's Hospital, Guangzhou 510180, China;
2 Cardiothoracic Surgery Department, The First Affilliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China

Received:2018-03-06 Online:2018-07-20 Published:2021-11-29

摘要/Abstract

摘要： 目的分析KRAS基因突变在左半结肠、右半结肠癌分布几率的差异性。方法回顾性分析我院病理科分子实验室2015年到2017年间接收的537例结肠癌手术标本,用ARMS-PCR法进行了KRAS基因的检测,并对左右半结肠腺癌KRAS基因突变比例进行χ²检验,P＜0.05为差异有统计学意义。结果 537例中有187(34.82%)例为右半结肠腺癌,左半结肠腺癌有350(65.18%)例。KRAS基因突变检测阳性例数为240例,阳性率为44.69%;右半结肠腺癌KRAS基因突变阳性数为114例,阳性率为60.96%;左半结肠癌KRAS基因突变阳性数为126例,阳性率为36.00%。双侧结肠腺癌KRAS基因突变差异有统计学意义。结论右半结肠癌的患病例数低于左半结肠癌,右半结肠癌KRAS基因突变阳性率较左半结肠癌高,本研究为区分左右半结肠癌的分子靶向治疗提供了数据支持。

关键词: 左半结肠腺癌, 右半结肠腺癌, KRAS基因突变, ARMS-PCR

Abstract: Objective To analyze the differences distribution of KRAS gene mutations in the left hemi colon cancer and right hemi colon cancer. Methods Retrospectively to analyse the 537 colorectal cancer patients who were received by our molecular laboratory of pathology from 2015 to 2017, ARMS-PCR method was used to detect the KRAS gene. The ratio of KRAS gene mutation in left and right hemi colon cancer was tested by χ² test, P<0.05 was considered statistically significant. Results There were 187(34.82%) patients with right hemi colon cancer; and there were 350 (65.18%)patients with left half hemi colon cancer. The KRAS positive patients were 240 cases, which were 44.69% in total. The positive patients with right hemi colon cancer were 114 cases which had a higher rate of 60.96%, the left hemi colon cancer patients were 126 cases with a lower rate of 36.00%. Conclusion The number of right hemi colon cancer was lower than the number of left hemi colon cancer. And the KRAS gene mutation in right hemi colon cancer had more positive than left hemi colon cancer. This study provides a number aid for treating right and left colon cancer in the subsequent molecular targeted drug therapy.

Key words: Right hemicolon cancer, Left hemicolon cancer, KRAS gene, ARMS-PCR

李秀博, 杜洪, 王小拍, 李雯, 沈春辉. 537例结肠腺癌患者KRAS基因检测结果分析[J]. 广州医药, 2018, 49(4): 7-9.

LI Xiubo, DU Hong, WANG Xiaopai, LI Wen, SHEN Chunhui. Retrospectively analysis of 537 colonic adenocarcinoma patients with KRAS gene detect[J]. Guangzhou Medical Journal, 2018, 49(4): 7-9.

537例结肠腺癌患者KRAS基因检测结果分析

Retrospectively analysis of 537 colonic adenocarcinoma patients with KRAS gene detect

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