中国人群中RHAG基因序列的观察

doi:10.3969/j.issn.1000-8535.2018.01.003

广州医药 ›› 2018, Vol. 49 ›› Issue (1): 9-12.DOI: 10.3969/j.issn.1000-8535.2018.01.003

中国人群中RHAG基因序列的观察

陈文伟¹, 邵超鹏²

1 中山市古镇人民医院检验科(中山 528400);
2 深圳市大学附属第一医院输血科(深圳 518000)

收稿日期:2017-09-30 出版日期:2018-01-20 发布日期:2021-12-01
通讯作者: 陈文伟,E-mail:401753338@qq.com
基金资助:
中山市卫计局基金项目(2016J264)

RHAG gene sequences in the Chinese population

CHEN Wenwei¹, SHAO Chaopeng²

1 Department of Clinical Laboratory, Guzhen People's Hospital of Zhongshan,Zhongshan 528400,China
2 Department of Blood Transfusion, The First Affiliated Hospital of Shenzhen University, Shenzhen 518000,China

Received:2017-09-30 Online:2018-01-20 Published:2021-12-01

摘要/Abstract

摘要： 目的探讨中国人群中不同Rh血型表型的RHAG基因序列。方法用血清学方法检测20例中国人群中不同Rh血型表型[包括了15例Rh(D)阳性样本和5例Rh(D)阴性样本,Rh(D)阳性样本中包含5例RhCcEe抗原弱表达、5例弱D和5例正常Rh(D)阳性样本],依据RHAG基因序列的特异性,设计10对特异性引物扩增20例样本的RHAG基因第1~10外显子,确认条带符合后送公司纯化并测序。结果 20例不同Rh血型表型的样本在统一PCR条件下均扩出RHAG基因1～10外显子,扩增产物测序结果与标准序列对比一致。结论了解中国人群中RHAG基因的多态性需观察更多有意义的样本。

关键词: 弱表达, RHAG基因, 序列分析, 表型

Abstract: Objective To explore the RHAG gene sequence of different Rh phenotypes in Chinese population. Methods We detected different Rh phenotypes in 20 cases of Chinese population using serological tests including 15 cases of Rh (D) positive samples and 5 cases of Rh (D) negative samples. Rh (D) positive samples included 5 cases of RhCcEe antigen weak expression, 5 cases of weak D and 5 cases of normal Rh (D) positive samples. According to specific RHAG gene sequence, 10 pairs of specific primers were designed to amplify 10 exon sequences of RHAG gene. Amplification products confirmed by electrophoresis, then the PCR products were purified and sequencing in a company. Results The exon 1~10 of RHAG gene in 20 cases of Chinese population coding region was sequenced, and the results were consistent with the sequence of GenBank standard. Conclusion More significant samples should be observed to study polymorphism of RHAG gene in Chinese population.

Key words: Weak expression, RHAG gene, Sequence analysis, Phenotype

陈文伟, 邵超鹏. 中国人群中RHAG基因序列的观察[J]. 广州医药, 2018, 49(1): 9-12.

CHEN Wenwei, SHAO Chaopeng. RHAG gene sequences in the Chinese population[J]. Guangzhou Medical Journal, 2018, 49(1): 9-12.

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中国人群中RHAG基因序列的观察

RHAG gene sequences in the Chinese population

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