异鼠李素对LPS诱导下THP-1细胞炎症因子释放的影响

doi:10.3969/j.issn.1000-8535.2017.05.003

广州医药 ›› 2017, Vol. 48 ›› Issue (5): 9-13.DOI: 10.3969/j.issn.1000-8535.2017.05.003

异鼠李素对LPS诱导下THP-1细胞炎症因子释放的影响

陈伟明, 熊旭明

广州市第一人民医院(广州 510180)

收稿日期:2017-04-26 发布日期:2021-12-01
通讯作者: 熊旭明,E-mail:xiongxuming9@126.com

The influence of the isorhamnetion on the releasing of inflammatory factor cytokines of the THP-1 cells stimulated by LPS

CHEN Weiming, XIONG Xuming

Guangzhou First People's Hospital,Guangzhou 510180,China

Received:2017-04-26 Published:2021-12-01

摘要/Abstract

摘要： 目的了解异鼠李素对LPS刺激下THP-1细胞炎症因子IL-6、MCP-1、TNF-α释放的影响及其抗炎特征。方法用PI单染色法检测其细胞存活率;用ELISA法检测THP-1细胞炎症因子IL-6、MCP-1、TNF-α释放。结果不同浓度的LPS均能使炎症因子IL-6释放增高,且24 h及48 h间水平无差异,但随着LPS刺激浓度的增高,细胞的坏死数量也会随之升高;不同浓度的异鼠李素能在不同时间点不同程度地抑制炎症因子MCP-1、TNF-α、IL-6的释放。结论异鼠李素能抑制LPS刺激下THP-1细胞炎症因子IL-6、TNF-α、MCP-1的释放,并呈浓度依赖性。

关键词: 异鼠李素, 脂多糖, 炎症因子, 抗炎反应, 单核细胞

Abstract: Objectives Our study is aim to investigate the influence of the isorhamnetin on the releasing of inflammatory factor cytokines of the THP-1 cells. Methods PI single staining method was used to detect the cell survival rate. ELISA was used to detecte the concentrations of inflammatory cytokines(MCP-1、IL-6、TNF-α). Results The concentration of IL-6 in THP-1 cells were increased after stimulated with LPS and there no difference between the 24 h group and 48 h group. The survival rate of THP-1 cells decreased as the concentration of LPS increased.The isorhamnetin in different concentration could inhibit the secretion of inflammatory cytokines in different time. Conclusion It is found in our study that isorhamnetin may inhibit the secretion of MCP-1, IL-6, TNF-α in THP-1 cells stimulated by LPS in a concentration dependent way.

Key words: Isorhamnetion, Lipopolysaccharides, Inflammatory cytokines, Anti-inflammtion, Mononuclear cell

陈伟明, 熊旭明. 异鼠李素对LPS诱导下THP-1细胞炎症因子释放的影响[J]. 广州医药, 2017, 48(5): 9-13.

CHEN Weiming, XIONG Xuming. The influence of the isorhamnetion on the releasing of inflammatory factor cytokines of the THP-1 cells stimulated by LPS[J]. Guangzhou Medical Journal, 2017, 48(5): 9-13.

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异鼠李素对LPS诱导下THP-1细胞炎症因子释放的影响

The influence of the isorhamnetion on the releasing of inflammatory factor cytokines of the THP-1 cells stimulated by LPS

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