自噬对鼻咽癌细胞CNE2放疗敏感性的调节作用

doi:10.3969/j.issn.1000-8535.2016.03.002

广州医药 ›› 2016, Vol. 47 ›› Issue (3): 4-7.DOI: 10.3969/j.issn.1000-8535.2016.03.002

自噬对鼻咽癌细胞CNE2放疗敏感性的调节作用

李宝秀¹, 方喜生¹, 张敏², 毛海波¹, 关明媚¹, 刘国龙¹

1 广州医科大学附属广州市第一人民医院肿瘤科(广州 510180)
2 广东药科大学公共卫生学院(广州 510310)

收稿日期:2016-03-25 出版日期:2016-05-20 发布日期:2021-11-30
通讯作者: 刘国龙,E-mail:liugl@fimmu.com
基金资助:
2012年广东省科技计划基金(01078650166731031);2011年度广东省医学科研基金(A2011469) ;2011年度广州市医药卫生科技一般引导项目(201102A213075)

The regulatory effects of autophagy to the CNE2 cells radio-sensitization

Li Baoxiu, Fang Xisheng, Zhang Min, Mao Haibo, Guan Mingmei, Liu Guolong

Li Baoxiu, Fang Xisheng, Mao Haibo, et al. Department of Oncology, Guangzhou First People's Hospital, Guangzhou MedicalUniversity, Guangzhou 510180, China
Zhang Min. School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China

Received:2016-03-25 Online:2016-05-20 Published:2021-11-30

摘要/Abstract

摘要： 目的探讨自噬激活剂和自噬抑制剂分别对鼻咽癌细胞CNE2放疗敏感性的影响。方法利用RNA干扰技术使atg5基因沉默,构建自噬抑制细胞模型后,与雷帕霉素、氯喹分别处理的两组细胞一起,每天以X射线5Gy照射细胞,连续8天观察各组细胞的生长状况,并设置对照组。以MTT法及克隆集落形成法检测其细胞活力,用流式细胞仪分析其细胞周期。结果与对照组相比,其他三组细胞存活率、克隆形成率、照射后存活率均显著降低(P<0.05);细胞周期检测除对照组外其他三组细胞集中在G0/G1期,其他两个时期比G0/G1期相对较少。结论自噬抑制剂与激活剂和atg5沉默均能为CNE2放疗增敏,然而自噬激活剂的增敏效果好于其他,为增敏放疗提供实验依据,开辟新的放疗增敏途径。

关键词: 自噬, 鼻咽癌, 放疗敏感性, 雷帕霉素, 氯喹

Abstract: Objective This study aimed to investigate the autophagy activators and inhibitors effects in nasopharyngeal CNE2 cells radiotherapy sensitization. Methods Atg5 gene silencing by RNA interference technology, two groups of cell autophagy inhibition were built by rapamycin and chloroquine respectively. Then 5Gy x-ray irradiation of cells was taken every day, after 8 days in a row in each group of cell growth and setting a control group. The cell viability was clonaled colony formation by MTT method assay and cell cycle by flow cytometry analysis. Results The three cell group survival rate, colony-forming rate and survival after irradiation were significantly lower (P<0.05) than the control group. Detection of cell cycle in addition to control three other groups concentrated in the G0/G1 period.That of two other periods was relatively fewer than that of the G0/G1 period. Conclusion Autophagy inhibitors, activators and atg5 silence improved the radio-sensitization to CNE2. The autophagy activator group improving the sensitivity was better than the others.This study provided evidence to sensitive radiotherapy, explored a new promising radiosensitization ways.

Key words: Aotophagy, CNE2, Radio-sensitization, Rapamycin, Chloroquine

李宝秀, 方喜生, 张敏, 毛海波, 关明媚, 刘国龙. 自噬对鼻咽癌细胞CNE2放疗敏感性的调节作用[J]. 广州医药, 2016, 47(3): 4-7.

Li Baoxiu, Fang Xisheng, Zhang Min, Mao Haibo, Guan Mingmei, Liu Guolong. The regulatory effects of autophagy to the CNE2 cells radio-sensitization[J]. Guangzhou Medical Journal, 2016, 47(3): 4-7.

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自噬对鼻咽癌细胞CNE2放疗敏感性的调节作用

The regulatory effects of autophagy to the CNE2 cells radio-sensitization

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