中山市部分地区金黄色葡萄球菌感染的临床分布及耐药基因研究

doi:10.3969/j.issn.1000-8535.2016.02.003

广州医药 ›› 2016, Vol. 47 ›› Issue (2): 7-9.DOI: 10.3969/j.issn.1000-8535.2016.02.003

中山市部分地区金黄色葡萄球菌感染的临床分布及耐药基因研究

林颜玉, 陈文伟, 王柏旺, 卢玉平

广东省中山市古镇人民医院检验科(中山 528421)

收稿日期:2015-11-18 出版日期:2016-03-20 发布日期:2021-11-30
通讯作者: E-mail:levyhan@163.com
基金资助:
中山市科技计划资助项目(2014AIFC058)

Clinical distribution and drug resistance gene of meticillin-resistant staphylococcus aureus infections in parts of Zhongshan city

Lin Yanyu, Chen Wenwei, Wang Bowang, et al

Zhongshan Guzhen People's Hospital, Zhongshan 528421, China

Received:2015-11-18 Online:2016-03-20 Published:2021-11-30

摘要/Abstract

摘要： 目的了解中山市7家医院金黄色葡萄球菌感染的临床分布,并对耐药基因进行检测,为临床经验治疗金黄色葡萄球菌感染提供用药及分子生物学依据。方法收集2015年1月—2015年6月中山市7家医院分离到的金黄色葡萄球菌,使用ATB半自动细菌鉴定及药敏分析仪(法国梅里埃)对分离到的菌株进行鉴定及药敏试验,使用PCR技术对耐甲氧西林金黄色葡萄球菌(MRSA)的耐药基因进行检测。结果 7家医院共分离到89株金黄色葡萄球菌,其中MRSA检出33株,检出率为37.1%。金黄色葡萄球菌主要来源于呼吸内科(32株,36.0%)、骨科(20株,22.5%),主要分离自痰(41株,46.1%),伤口分泌物(16株,18%),对万古霉素、替考拉宁、奎奴普丁/达福普丁、复方新诺明、左氧氟沙星、诺氟沙星具有较高敏感性,MRSA对常用抗菌药物耐药率高于甲氧西林敏感金黄色葡萄球菌。共有32株MRSA检出bla_mecA基因,检出率为97%。结论 MRSA耐药情况较为严峻,临床科室应根据微生物培养报告合理使用抗菌药物。bla_mecA基因在MRSA检出较高,是MRSA主要的耐药机制。

关键词: 耐甲氧西林金黄色葡萄球菌, 抗菌药物, 耐药性, bla_mecA基因

Abstract: Objective To analyze clinical distribution of Staphylococcus aureus infections from 7 hospitals in Zhongshan city, as well as to provide basis of empirical treatment and molecular biology for Staphylococcus aureus infections. Methods Staphylococcus aureus were collected from January 2015 to June 2015 in Zhongshan city, and then the strains were identified and tested antibiotic susceptibility by using ATB semiautomatic analyzer(Merieux). Resistance gene of methicillin-resistant Staphylococcus aureus(MRSA) was detected by polymerase chain reaction. Results 89 strains of Staphylococcus aureus were isolated from 7 hospitals and with prevalence of 33 strains of MRSA. Of all strains, 32(36.0%) were isolated from respiratory medicine and 20(22.5%) from orthopedics. 41(46.1%) strains of Staphylococcus aureus were isolated from sputum and 16(18.0%) from wound secretion. 89 strains of Staphylococcus aureus had highly susceptibility to vancomycin, teicoplanin, quinupristin/dalfopristin, cotrimoxazole, levofloxacin, norfloxacin. Resistance rates to commonly used antimicrobial drugs of MRSA were significantly higher than methicillin-sensitive. A total of 32 MRSA were detected carrying bla_mecA gene with the detection rate of 97%. Conclusion Clinical departments should be based on microbial culture report for rational use of antibiotics because of MRSA with more serious drug resistance. The gene of bla_mecA is the main mechanism of resistance for MRSA.

Key words: Meticillin-resistant Staphylococcus aureus, Antibiotic, Drug resistance, bla_mecA gene

林颜玉, 陈文伟, 王柏旺, 卢玉平. 中山市部分地区金黄色葡萄球菌感染的临床分布及耐药基因研究[J]. 广州医药, 2016, 47(2): 7-9.

Lin Yanyu, Chen Wenwei, Wang Bowang, et al. Clinical distribution and drug resistance gene of meticillin-resistant staphylococcus aureus infections in parts of Zhongshan city[J]. Guangzhou Medical Journal, 2016, 47(2): 7-9.

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中山市部分地区金黄色葡萄球菌感染的临床分布及耐药基因研究

Clinical distribution and drug resistance gene of meticillin-resistant staphylococcus aureus infections in parts of Zhongshan city

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