高龄肝脏与低龄肝脏生理差异的实验性研究

doi:10.3969/j.issn.1000-8535.2015.02.003

广州医药 ›› 2015, Vol. 46 ›› Issue (2): 7-12.DOI: 10.3969/j.issn.1000-8535.2015.02.003

高龄肝脏与低龄肝脏生理差异的实验性研究

黄宇¹, 黄迪^1,2, 翁杰锋^1,2, 张帅^1,2, 张强¹, 古维立^1,2

1 广州医科大学附属广州市第一人民医院肝胆外科 (广州 510180)
2 广州消化疾病中心(广州 510180)

收稿日期:2015-01-10 发布日期:2021-11-30
通讯作者: 古维立,E-mail:lili-6423@163.com
基金资助:
广东省科技计划项目(2011B061300024,2013B021800057);广东省自然基金项目(10151006001000013);广州市医药卫生科技一般引导项目(20131A011029,20141A010003);广州市科技和信息化局科普专项项目(2014KP000086)

Experimental of physiological differences between elderly and younger livers in rats

Huang Yu, Huang Di, Wong Jiefeng, Zhang Shuai, et al

Department of Thyroid and Breast Surgery, General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzou 510180,China;
Department of Hepatobiliary Surgery, General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180,China

Received:2015-01-10 Published:2021-11-30

摘要/Abstract

摘要： 目的探讨生理情况下高龄肝脏与低龄肝脏的区别,寻找可以区分高龄肝脏与低龄肝脏的指标。方法取6周龄SD大鼠和9月龄以上退役SD大鼠各5只,采用超声弹性成像检测肝脏硬度、全自动生化检测仪检测血清学指标、H&E染色观察肝脏形态结构、Sirius Red染色及Masson染色检测胶原纤维的沉积、免疫组化SP法检测TGF-β1、p16^INK4a、SMP-30蛋白的表达。结果高龄组和低龄组之间血清学指标、胶原纤维沉积及TGF-β蛋白、p16^INK4a蛋白的表达无差异;超声弹性成像检测低龄组Vs值为(1.21±0.09)m/s,高龄组为(1.32±0.05)m/s(P=0.033);SMP-30蛋白低龄组IOD值为138244.988±51286.257,高龄组为116240.170±35017.936(P=0.007)。结论高龄大鼠与低龄大鼠肝脏的硬度及SMP-30蛋白的表达存在差异,随着年龄的增加肝脏硬度增大,SMP-30蛋白表达下降。肝脏硬度与SMP-30蛋白可作为区分高龄肝脏与低龄肝脏的指标。

关键词: 高龄, 肝移植, SMP-30, 超声弹性成像

Abstract: Objective To investigate the differences between elderly and younger liver. Methods In accordance with the age of the SD rats into two groups: younger group (Group Y, 6 weeks, n=5) and elderly group (Group O, 40 weeks or more, n=5). Data were compared by using ultrasound elasticity imaging to detect liver stiffness, automatic biochemical detector to gauge serum indexes, H&E staining to observe the liver morphological structure, Sirius Red staining and Masson staining to assay the collagen fibers deposition, Immunohistochemistry to identify the expression of TGF-β₁, p16^INK4a and SMP-30 protein. Results Serum indexes, collagen deposition, TGF-β₁ and p16^INK4a protein expression were no statistically significant difference between two groups. The Vs value was (1.21±0.09) m/s in Group Y and (1.32±0.05) m/s in Group O (P=0.033). and the IOD value of SMP-30 protein between Group Y and Group O were 138244.988±51286.257 and 116240.170±35017.936 (P=0.007). Conclusion The degree of liver stiffnessnd and SMP-30 protein in elderly and younger liver are different.Increased the degree of liver stiffness and decreased the expression of SMP-30 protein in the elderly SD rats. Liver stiffness and SMP-30 protein could be used as indicators to distinguish between elderly and younger liver.

Key words: Elderly, Liver transplantation, SMP-30, Ultrasound elastography

黄宇, 黄迪, 翁杰锋, 张帅, 张强, 古维立. 高龄肝脏与低龄肝脏生理差异的实验性研究[J]. 广州医药, 2015, 46(2): 7-12.

Huang Yu, Huang Di, Wong Jiefeng, Zhang Shuai, et al. Experimental of physiological differences between elderly and younger livers in rats[J]. Guangzhou Medical Journal, 2015, 46(2): 7-12.

参考文献

[1] Fausto N. Liver regeneration and repair: hepatocytes, progenitor cells, and stem cells[J]. Hepatology, 2004, 39(6): 1477-1487.
[2] Jimenez-Romero C, Caso MO, Cambra MF, et al.Using old liver grafts for liver transplantation: where are the limits[J]. World J Gastroenterol, 2014, 20(31): 10691-10702.
[3] Zhou S F, Mo FR, Bin YH, et al. Serum immunoreactivity of SMP30 and its tissues expression in hepatocellular carcinoma[J]. Clin Biochem, 2011, 44(4): 331-336.
[4] 胡志文, 黄迪, 张帅,等. 超声弹性成像监测下大鼠肝纤维化模型的制备[J]. 广东医学, 2012, 33(16): 2376-2378.
[5] Andreollo NA, Santos EF, Araujo M R, et al. Rat's age versus human's age: what is the relationship[J]. Arq Bras Cir Dig, 2012, 25(1): 49-51.
[6] 赵夏夏, 吴永胜, 苗英,等. 不同年龄段人群正常肝脏弹性成像定量的相关性分析[J]. 西北国防医学杂志, 2012, 33(5): 539-541.
[7] 胡志文, 黄迪, 罗嘉伦,等. 超声弹性成像在肝脏纤维化诊断中的研究概况[J]. 广州医药, 2012, 43(6): 54-56.
[8] 黄迪, 古维立, 胡志文,等. 大鼠肝纤维化模型建立及肝纤维化的检测[J]. 广州医药, 2012, 43(5): 56-60.
[9] 袁翠云, 付敏. 联合检测血清肝纤维化指标在慢性肝病治疗中的价值[J]. 哈尔滨医药, 2002, 22(6): 11-12.
[10] 王长友, 张惠彬. 肝纤维化的血清学检查[J]. 哈尔滨医药, 2003, 23(1): 60-62.
[11] Fujita T. Senescence marker protein-30 (SMP30): structure and biological function.[J]. Biochem Biophys Res Commun, 1999, 254(1): 1-4.
[12] 莫发荣, 李颀, 何少健,等. 衰老标记蛋白-30的表达与肝疾病的关系[J]. 解剖学报, 2007(5): 589-592.
[13] Fujita T, Uchida K, Maruyama N. Purification of senescence marker protein-30 (SMP30) and its androgen-independent decrease with age in the rat liver.[J]. Biochim Biophys Acta, 1992, 1116(2): 122-128.
[14] 郭晓强, 刘卫, 张如春. 抑癌蛋白p16^INK4a:一种新的衰老调控因子[J]. 生命的化学, 2007, 27(2): 144-146.

高龄肝脏与低龄肝脏生理差异的实验性研究

Experimental of physiological differences between elderly and younger livers in rats

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

本文评价

[1]	郑素娟, 冯彩莲, 樊建凤. 高龄呼吸道感染患者病原菌检验以及耐药性监测结果分析[J]. 广州医药, 2021, 52(6): 83-86.
[2]	黄文宇, 林永汉, 熊小英. 孕中后期中低等强度有氧运动对高龄孕妇产前生活质量和母婴结局的影响探讨[J]. 广州医药, 2020, 51(5): 66-70.
[3]	黄月娥, 李志根, 李丹莉, 蒋妍琳. 高龄患者植入静脉输液港并发感染相关影响因素分析[J]. 广州医药, 2020, 51(4): 71-74.
[4]	张秀玲. 实时超声弹性成像技术评估脑卒中患者颈动脉斑块稳定性的应用研究[J]. 广州医药, 2020, 51(1): 14-18.
[5]	粟琼瑜, 刘洁珍, 蔡琼, 邝玉群. 个体化健康管理对超高龄结直肠癌患者希望水平的影响[J]. 广州医药, 2017, 48(3): 75-77.
[6]	林军华, 赵善明, 陈进宇, 郭志荣, 张健荣. 经皮微创应用股骨近端解剖锁定钢板治疗高龄骨质疏松粗隆间骨折[J]. 广州医药, 2015, 46(4): 50-52.