高压氧对FVB小鼠旷场行为的影响

doi:10.3969/j.issn.1000-8535.2018.03.001

广州医药 ›› 2018, Vol. 49 ›› Issue (3): 1-4.DOI: 10.3969/j.issn.1000-8535.2018.03.001

• 论著 • 下一篇

高压氧对FVB小鼠旷场行为的影响

罗朝文¹, 成金玲¹, 萧丹贤¹, 张楚瑜¹, 冯嘉欣¹, 陈盛强², 胡楠², 黄梅秀²

1 广州医科大学金域检验学院(广州 511436)
2 广州医科大学附属第二医院(广州 510260)

收稿日期:2018-02-11 出版日期:2018-05-20 发布日期:2021-12-01
通讯作者: 黄梅秀,E-mail: 1040930128@qq.com
基金资助:
国家自然科学基金(31771327);广东省科技计划项目 (2015A0030302090);广州市教育局创新学术团队项目(1201610032);广州市卫生局科技项目 (20151A010129) (20151A011080);广州医科大学大学生科技创新项目(2017A059)

The effect of hyperbaric oxygenation on behaviors of FVB mice in the open field test

LUO Chaowen¹, CHENG Jinling¹, XIAO Danxian¹, ZHANG Chuyu¹, FENG Jiaxin¹, CHEN Shengqiang², HU Nan², HUANG Meixiu²

1 King Med College of Laboratory Medicine, Guangzhou Medical University, Guangzhou 511436, China
2 The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China

Received:2018-02-11 Online:2018-05-20 Published:2021-12-01

摘要/Abstract

摘要： 目的探讨高压氧对FVB小鼠旷场行为的影响。方法对9只4周龄高压氧组FVB小鼠进行2个疗程共21天高压氧干预;对13只4周龄对照组FVB小鼠不做任何处理,正常饲养。21天后对2组小鼠进行旷场实验,观察高压氧对FVB小鼠旷场行为的影响。结果高压氧组小鼠在中央区停留时间(3.73±3.69,17.97±6.82,t=-5.564,P=0.000)和运动路程较对照组小鼠减少(189.65±199.32,525.67±244.22,t=-3.298,P=0.040),在外周区停留时间增加(296.07±3.69,281.79±6.79,t=5.597,P=0.000);进入中央区、外周区次数及穿越各区总次数都减少(3.11±3.10,8.20±4.80, t=-2.770,P=0.014;3.11±3.10,9.10±4.79,t=-3.264,P=0.005;6.22±6.20,17.30±9.59,t=-3.018,P=0.008);2组小鼠总路程和平均速度无差异(8766.57±3362.90,8320.47±1692.47, t=0.359,P=0.726;73.05±28.02,69.34±14.10,t=0.359,P=0.726)。结论高压氧改变FVB小鼠的旷场行为的表型,使小鼠趋避性增加,对环境的认知能力及焦虑程度增强,探索性下降。

关键词: 高压氧, FVB小鼠, 旷场, 行为学

Abstract: Objective To explore the effect of hyperbaric oxygenation(HBO)on behaviors of FVB mice in the open field test. Methods 9 mice of four-week-old in the HBO group were treated with hyperbaric oxygenation for a total of 21 days during two courses while 13 control mice of four-week-old did not do any treatment. After 21-day normal breeding, the mice of two groups were tested with open field test. Then, researchers observed the effect of hyperbaric oxygenation on mice behaviors after open field test. Results Compared to 13 control mice, residence time (3.73±3.69,17.97±6.82,t=-5.564,P=0.000) and the range of movement (189.65±199.32,525.67±244.22,t=-3.298,P=0.040)in central region decreased while the residence time in external region increased (296.07±3.69,281.79±6.79,t=5.597,P=0.000). The times of entering the central region and external region and crossing both of the regions decreased (3.11±3.10,8.20±4.80, t=-2.770,P=0.014;3.11±3.10,9.10±4.79,t=-3.264,P=0.005;6.22±6.20,17.30±9.59,t=-3.018,P=0.008). There was no difference in the total range of movement and the average velocity (8766.57±3362.90,8320.47±1692.47, t=0.359,P=0.726;73.05±28.02,69.34±14.10,t=0.359,P=0.726). Conclusion Hyperbaric oxygenation may change the behaviors of FVB mice in open field test to enhance its phobotaxis, anxiety degree and the cognitive ability in environment and inhibiting its exploration ability.

Key words: Hyperbaric oxygenation, FVB mice, Open field test, Behavior

罗朝文, 成金玲, 萧丹贤, 张楚瑜, 冯嘉欣, 陈盛强, 胡楠, 黄梅秀. 高压氧对FVB小鼠旷场行为的影响[J]. 广州医药, 2018, 49(3): 1-4.

LUO Chaowen, CHENG Jinling, XIAO Danxian, ZHANG Chuyu, FENG Jiaxin, CHEN Shengqiang, HU Nan, HUANG Meixiu. The effect of hyperbaric oxygenation on behaviors of FVB mice in the open field test[J]. Guangzhou Medical Journal, 2018, 49(3): 1-4.

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高压氧对FVB小鼠旷场行为的影响

The effect of hyperbaric oxygenation on behaviors of FVB mice in the open field test

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