Bax抑制肽对新生大鼠肠组织的保护作用

doi:10.3969/j.issn.1000-8535.2015.06.004

广州医药 ›› 2015, Vol. 46 ›› Issue (6): 12-17.DOI: 10.3969/j.issn.1000-8535.2015.06.004

Bax抑制肽对新生大鼠肠组织的保护作用

崔伟伦¹, 冯帼¹, 刘素丽¹, 谭洪辉¹, 叶炳飞², 张炼³

1 广州市番禺区中心医院(广州 511486);
2 广州医科大学实验动物中心(广州 510182);
3 广州市妇女儿童医疗中心(广州 510623)

收稿日期:2015-08-17 发布日期:2021-11-30
基金资助:
广东省科技计划项目[粤科规划字(2013)137号]

Protection of Bax inhibitory peptides to newborn rat intestinal tissue

Cui Weilun, Feng Guo, Lin Suli, Ye Bingfei, Zhang Lian, et al

Guangzhou panyu district central hospital Guangzhou 511486, China;
The laboratory animal center, Guangzhou medical university Guangzhou 510182,China;
The Guangzhou women and children medical center Guangzhou 510623, China

Received:2015-08-17 Published:2021-11-30

摘要/Abstract

摘要： 目的观察Bax抑制因子(Bax-inhibiting peptides,BIP)对肠上皮细胞的保护作用,并探讨其作用机制。方法建立1日龄新生大鼠的坏死性小肠结肠炎模型。BIP于建模前腹腔内注射,建模后分别检测各组肠组织病理,并分别用流式细胞仪检测各组肠细胞凋亡率,western blot法检测bax下游凋亡蛋白细胞色素C、caspase 9和caspase 3含量。结果与NEC组及Bax10 μg组比较,bax50 μg和100 μg可减轻肠上皮损伤,减少肠细胞凋亡率,降低bax下游凋亡蛋白细胞色素C、caspase9和caspase3含量。结论一定剂量的Bax抑制肽可通过降低活性Caspase-3及CytC蛋白释放,保护线粒体膜,抑制肠上皮细胞调亡,发挥对肠上皮细胞的保护作用。

关键词: Bax抑制肽, 肠上皮细胞凋亡, 坏死性小肠结肠炎, 保护作用

Abstract: Objective To investigate the protective effects of Bax-inhibiting peptides(BIP) on intestinal epithelial cells and to explore its mechanism. Methods The model of neonatal necrotizing enterocolitis in 1 day neonatal rats were established. Before establishing the model, BIP was intraperitoneal injected to the rats. The pathological of intestinal tissue were detected respectively, the intestinal cell apoptosis rate were detected by flow cytometry, the levels of downstream apoptosis proteins of Bax were detected by Western blotting respectively. Results Compared to NEC and Bax10μg group, bax50μg and 100 μg can significantly reduce the intestinal epithelial damage and intestinal cell apoptosis rate and can decrease the levels of cytochrome C, caspase-9 and caspase-3, downstream apoptosis proteins of Bax,significantly. Conclusion A certain dose of Bax-inhibiting peptides can protect the mitochondrial membrane, inhibit the apoptosis of intestinal epithelial cells by reducing the level of Caspase-3 and CytC and play a protective effect on intestinal epithelial cells.

Key words: Bax-inhibiting peptides, The apoptosis of intestinal cell, Necrotizing enterocolitis, Protective effect

崔伟伦, 冯帼, 刘素丽, 谭洪辉, 叶炳飞, 张炼. Bax抑制肽对新生大鼠肠组织的保护作用[J]. 广州医药, 2015, 46(6): 12-17.

Cui Weilun, Feng Guo, Lin Suli, Ye Bingfei, Zhang Lian, et al. Protection of Bax inhibitory peptides to newborn rat intestinal tissue[J]. Guangzhou Medical Journal, 2015, 46(6): 12-17.

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Bax抑制肽对新生大鼠肠组织的保护作用

Protection of Bax inhibitory peptides to newborn rat intestinal tissue

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