脂溶性HHRT与水溶性MMC、5-Fu对人Tenon's囊成纤维细胞抑制作用的研究

doi:10.3969/j.issn.1000-8535.2015.03.002

广州医药 ›› 2015, Vol. 46 ›› Issue (3): 4-7.DOI: 10.3969/j.issn.1000-8535.2015.03.002

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脂溶性HHRT与水溶性MMC、5-Fu对人Tenon's囊成纤维细胞抑制作用的研究

冷云霞, 张柳, 陈文俐, 黄雄飞, 杨为中

广州市第一人民医院(广州 510180)

收稿日期:2015-01-04 出版日期:2015-05-20 发布日期:2021-11-30
通讯作者: 杨为中,E-mail:2457216451@qq.com
基金资助:
广东省自然科学基金博士启动项目(S2013040012562);广州市科技局基金项目(11C33150706)

Study on the inhibitory effects of Homoharringtonine, Mitomycin-C and 5-Fluorouracil and cultured Human Tenon's fibroblasts

Leng Yunxia, Zhang Liu, Chen Wenli, et al

Guangzhou First People's Hospital, Guangzhou 510180, China

Received:2015-01-04 Online:2015-05-20 Published:2021-11-30

摘要/Abstract

摘要： 目的比较脂溶性高杉尖三酯碱(HHRT)与水溶新丝裂霉素C(MMC)和5-氟尿嘧啶(5-Fu)三种药物对人Tenon's囊成纤维细胞(HTFs)增殖率的抑制情况。方法采用冻存的人眼Tenon's囊成纤维细胞,常规复苏体外培养稳定后,接种于96孔板,分别加入不同浓度HHRT、MMC及5-Fu,继续培养;采用MTT法于加药后24 h、48 h、72 h检测不同浓度HHRT、MMC及5-Fu对体外培养的人眼Tenon囊成纤维细胞增值率的抑制情况。结果一定浓度的HHRT、MMC及5-Fu,对HTFs的抑制作用呈现不同的剂量和时间依赖性,在药物浓度达到相应程度作用72 h后三者对HTFs的最大抑制率均可达70%以上,且组间对比差异有统计学意义。结论三种药物均对人眼Tenon's囊成纤维细胞增殖具有明显抑制作用,其最大抑制率均可达70%以上,三种药物对成纤维细胞抗增殖效应的时间浓度依赖性有所差别。

关键词: 高三尖杉酯碱/HHRT, 丝裂霉素C/MMC, 5-氟尿嘧啶/5-Fu, 人Tenon's囊成纤维细胞/HTFs, 比色法/MTT

Abstract: Objective To investigate and compare the inhibitory effects of Mitomycin C(MMC), 5-Fluorouracil(5-Fu) and Homoharringtonine(HHRT) on cultured Human Tenon's fibroblasts(HTFs) proliferation. Methods Fibroblasts were recovered and cultured from cell bank.Fibroblasts were treated with different concentration of MMC, 5-Fu and HHRT for 48h and 72h respectively. The proliferation of fibroblasts was detected using MTT assay. Results Fibroblasts were cultured in MMC, 5-Fu or HHRT demonstrated a dose-dependent and time-dependent inhibition of fibroblasts proliferation.The maximum inhibition rate was found when HTFs were treated with different concentration of MMC, 5-Fu or HHRT for 72h. And the three agents maximum inhibition rates were all more than 70%. The differences of proliferation in control and treated groups was statistically significant(P<0.01). Conclusion The three agents had inhibitory effect on HTFs. And the three agents maximum inhibition rates were all more than 70%. The inhibitory effects of MMC,5-Fu and dexamethasone on fibroblast proliferationwas MMC>HHRT>5-Fu.

Key words: Mitomycin C, 5-Fluoromaeil, Homoharringtonine, Human Tenon's fibroblasts, Methyl thiazlyl tetrazolium

冷云霞, 张柳, 陈文俐, 黄雄飞, 杨为中. 脂溶性HHRT与水溶性MMC、5-Fu对人Tenon's囊成纤维细胞抑制作用的研究[J]. 广州医药, 2015, 46(3): 4-7.

Leng Yunxia, Zhang Liu, Chen Wenli, et al. Study on the inhibitory effects of Homoharringtonine, Mitomycin-C and 5-Fluorouracil and cultured Human Tenon's fibroblasts[J]. Guangzhou Medical Journal, 2015, 46(3): 4-7.

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脂溶性HHRT与水溶性MMC、5-Fu对人Tenon's囊成纤维细胞抑制作用的研究

Study on the inhibitory effects of Homoharringtonine, Mitomycin-C and 5-Fluorouracil and cultured Human Tenon's fibroblasts

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