Beckman AU5811生化分析仪交叉污染实验设计与应用

doi:10.3969/j.issn.1000-8535.2015.02.005

广州医药 ›› 2015, Vol. 46 ›› Issue (2): 16-18.DOI: 10.3969/j.issn.1000-8535.2015.02.005

Beckman AU5811生化分析仪交叉污染实验设计与应用

胡远明¹, 熊石龙¹, 石凌波², 杨玉娟¹, 范灿波¹, 王治伟¹

1 广州医科大学附属第四医院检验科(广州 511447);
2 广东祈福医院检验科(广州 511495)

收稿日期:2015-01-27 发布日期:2021-11-30
通讯作者: 胡远明,E-mail:qzwxhym@163.com
基金资助:
国家高技术研究发展计划(863计划)子课题项目(2014AA022304)

The Design and application of beckman AU5811 automatic biochemical analyzer's cross contamination experiments

Hu Yuanming, Xiong Shilong, Shi Lingbo, Yang Yujuan, et al

Department of Clinical Laboratory, the fourth hospital affiliated to Guangzhou Medical University, Guangzhou 511447, China;
Department of Clinical Laboratory, Clifford Hospital of Guangdong, Guangzhou 511447, China

Received:2015-01-27 Published:2021-11-30

摘要/Abstract

摘要： 目的设计并验证适合Beckman AU5811全自动生化分析仪试剂针和搅拌棒的交叉污染分析方法,找到产生的原因并制定有效的污染避免措施。方法以反应盘内圈项目(ALT、TBIL、GGT、ALB、TBA、LDH1、CK、Cr、GLU、CO2、TC、HDL-C、APOA1、Ca 、Fe、Cu、HCY 、AFU、PA)作为实验项目,按设计的检测顺序进行交叉污染实验,各项目间的污染率以不超过95%～105%为判断标准。结果内圈项目中TC、HCL-C对TBA分别存在试剂针和搅拌棒的污染,Ca对Cr存在搅拌棒的污染,Cu对Fe存在试剂针的污染,GGT、ALB、LDH1对CO2都有搅拌棒的污染,TC、TBIL对Cu也存在搅拌棒的污染。结论采用该方法能有效快速地对Beckman AU5811生化分析仪上的所有项目进行交叉污染实验,确定交叉污染产生的关联项目并采取污染避免措施减少交叉污染,保证检验结果的准确。

关键词: 生化分析仪, 交叉污染, 实验设计

Abstract: Objective To design and verify an analytical method for the cross contamination of Beckman AU5811 automatic biochemical analyzer's reagent needle and stir bar and find the reasons, and make effective measures to avoid contamination. Methods Use the inner ring items (ALT,TBIL,GGT,ALB, TBA,LDH1,CK,Cr,GLU,CO2,TC,HDL-C,APOA1,Ca,Fe,Cu,HCY,AFU,PA) as theexperiment objects and do the cross contamination experiment according to the test order. The standard for the cross contamination level is limited to 95-105% among all items. Results TC and HCL-C were found to have contaminated TBA both in reagent needle and stir bar model, Ca contaminated Cr in the stir bar model; Cu contaminated Fe in the reagent needle model; GGT,ALB and LDH1 contaminated CO2 in stir bar model; TC and TBIL contaminated Cu in the stir bar model. Conclusion The method used in this experiment can effectively identify and analyze the cross contamination of all the items in Beckman AU5811 automatic biochemical analyzer. In addition, it can help to adopt corresponding measures to reduce cross contamination.

Key words: Biochemical Analyzer, Cross contamination, The experiment design

胡远明, 熊石龙, 石凌波, 杨玉娟, 范灿波, 王治伟. Beckman AU5811生化分析仪交叉污染实验设计与应用[J]. 广州医药, 2015, 46(2): 16-18.

Hu Yuanming, Xiong Shilong, Shi Lingbo, Yang Yujuan, et al. The Design and application of beckman AU5811 automatic biochemical analyzer's cross contamination experiments[J]. Guangzhou Medical Journal, 2015, 46(2): 16-18.

Beckman AU5811生化分析仪交叉污染实验设计与应用

The Design and application of beckman AU5811 automatic biochemical analyzer's cross contamination experiments

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