探讨肺动脉高压大鼠心肌损伤与氧化应激损伤的相关性研究

doi:10.3969/j.issn.1000-8535.2021.01.001

广州医药 ›› 2021, Vol. 52 ›› Issue (1): 1-5.DOI: 10.3969/j.issn.1000-8535.2021.01.001

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探讨肺动脉高压大鼠心肌损伤与氧化应激损伤的相关性研究

翟子敬^1,2, 秦文娟², 王臻^1,2, 郭雪婷², 王忠³, 芦桂林²

1 石河子大学,石河子大学医学院(石河子 832008)
2 石河子大学医学院第一附属医院超声医学科(石河子 832008)
3 石河子大学医学院第一附属医院心内科(石河子 832008)

收稿日期:2020-05-09 出版日期:2021-01-20 发布日期:2021-11-22
通讯作者: 芦桂林,E-mail: 690821295@qq.com
基金资助:
国家自然科学基金项目(81460075)

Study on the relationship between myocardial injury and oxidative stress injury in rats with pulmonary hypertension

ZHAI Zijing^1,2, QIN Wenjuan², WANG Zhen^1,2, GUO Xueting², WANG Zhong³, LU Guilin²

1 Shihezi University, Medical College of Shihezi University, Shihezi 832008, China
2 Department of Ultrasound Medicine, First Affiliated Hospital, School of Medicine Shihezi University, Shihezi 832008, China
3 Department of Cardiology, First Affiliated Hospital, School of Medicine Shihezi University, Shihezi 832008, China

Received:2020-05-09 Online:2021-01-20 Published:2021-11-22

摘要/Abstract

摘要： 目的探讨应用超声心动图评价肺动脉高压(PAH)致心肌损伤的临床价值及氧化应激损伤的相关性。方法 12周龄SD大鼠48只。随机均分为4组:空白对照组、NaCl对照组、PAH 2周组及PAH 4周组。建模后,采用超声检测相关参数。HE及Masson染色观察右心室的心肌细胞及胶原纤维分布情况,测定超氧化物歧化酶(SOD)活力,还原型谷胱甘肽(GSH)和丙二醛(MDA)水平,并评价其与超声参数的相关性。结果 PAH组大鼠超声相关参数均变化明显,且逐渐加重。HE及Masson染色显示心肌细胞增大,心肌间隙中的胶原纤维明显增多,且4周组较明显。PAH组大鼠心肌组织中的SOD活力及GSH水平较低,而MDA水平较高,有变化趋势,且与超声参数有显著相关性,差异均有统计学意义(P<0.05)。结论 PAH导致大鼠右心室心肌组织结构改变,同时引起氧化应激相关指标的变化。

关键词: 超声心动图, 肺动脉高压, 右心室, 大鼠, 氧化应激

Abstract: Objective To explore the clinical value of echocardiographic evaluation of myocardial injury caused by pulmonary hypertension (PAH) and the correlation of oxidative stress injury. Methods Forty-eight 12-week-old SD rats were collected. They were randomly divided into 4 groups: blank control group, NaCl control group, PAH 2-week group and PAH 4-week group. After modeling, ultrasound was used to detect relevant parameters. HE and Masson staining were used to observe the distribution of myocardial cells and collagen fibers in the right ventricle. Superoxide dismutase (SOD) activity, reduced glutathione (GSH) and malondialdehyde (MDA) levels were measured, and their correlations with ultrasound parameters were evaluated. Results The ultrasound-related parameters of rats in the PAH group changed significantly and gradually increased.HE and Masson staining showed that cardiac myocytes were enlarged and collagen fibers in myocardial interstices were increased, and it was more obvious in the 4-week group.In the PAH group, the SOD activity and GSH levels were lower, while the MDA levels were higher, and there was a trend of change, and there was a significant correlation with ultrasound parameters. The difference was statistically significant (P<0.05). Conclusion PAH causes changes in the myocardial tissue structure of the rat right ventricle, as well as changes in oxidative stress-related indicators.

Key words: Echocardiography, Pulmonary hypertension, Right ventricle, Rats, Oxidative stress

翟子敬, 秦文娟, 王臻, 郭雪婷, 王忠, 芦桂林. 探讨肺动脉高压大鼠心肌损伤与氧化应激损伤的相关性研究[J]. 广州医药, 2021, 52(1): 1-5.

ZHAI Zijing, QIN Wenjuan, WANG Zhen, GUO Xueting, WANG Zhong, LU Guilin. Study on the relationship between myocardial injury and oxidative stress injury in rats with pulmonary hypertension[J]. Guangzhou Medical Journal, 2021, 52(1): 1-5.

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探讨肺动脉高压大鼠心肌损伤与氧化应激损伤的相关性研究

Study on the relationship between myocardial injury and oxidative stress injury in rats with pulmonary hypertension

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