关键词: NXT629, 脂代谢, 肝胆结石, 成石饮食, PPAR-α

Abstract: Objective To explore the mechanism on NXT629 improves hepatolithiasis formation．Methods C57BL/6J mice were fed either a regular diet or a lithogenic diet（LD）,with the LD group receiving injections of PPAR-α inhibitor NXT629．Liver steatosis was analyzed via HE staining,hepatic lipid accumulation was detected by Oil Red O staining,and total cholesterol,triglycerides,phospholipids,total bile acids,cholesterol saturation index,low density lipoprotein cholesterol,and high density lipoprotein cholesterol levels in bile or serum were measured using assay kits．RT-qPCR was employed to determine the mRNA expression of ABCG5/8,CYP7A1,CYP7B1,PPAR-α,and ABCB11 in mouse liver tissues．Results The Results showed that NXT629 target PPAR-α to down-regulate the mRNA levels of ABCG5,ABCG8,and ABCB11 in the livers of LD-fed mice,while increasing the mRNA levels of CYP7A1 and CYP7B1,thereby reducing LD-induced hepatolithiasis formation and improving lipid metabolism disorders．Conclusions NXT629 can improve cholesterol gallstones by affecting the expression of genes related to lipid metabolism．

Key words: NXT629, lipid metabolism, hepatolithiasis, lithogenic diet, PPAR-α