关键词: 玻璃体腔注射, 玻璃毛细管, 小胶质细胞

Abstract: Objective To assess the safety and feasibility of employing an enhanced glass capillary,with a diameter of 0.08 mm,as an intraocular needle for intravitreal injections in experimental mice eyes．Methods Twelve male C57BL/6J mice,aged 6-8 weeks,were utilized in this investigation．Phosphate buffered saline（PBS）was administered via intravitreal injection into the left eye of each mouse（experimental group）,while the right eye received no special treatment（control group）．Six mice received an intraperitoneal injection of Evans blue immediately following intravitreal injection to detect retinal vessel leakage．The remaining six mice were euthanized 24 hours after intravitreal injection,and the retinas were subjected to immunofluorescence staining using a microglia-specific antibody to analyze morphological changes in microglia．Results In both the experimental and control groups,the ratio of vascular to peripheral fluorescence intensity was（4.45±0.30）and（4.51±0.24）,respectively．The number of microglia was（131.00±5.38）/mm² and（133.00±5.99）/mm²,the cell body area of microglia was 34.02（27.82,40.54）μm² and 34.70（26.09,40.54）μm²,and the branch length of microglia was 198.80（171.30,258.80）μm and 223.30（178.20,278.30）μm,respectively．There were no statistically differences observed in any of the above indicators between the two groups（all P>0.05）． Conclusions The use of this glass capillary,characterized by a narrower diameter,reduces tissue damage,demonstrates its potential to replace traditional injection needles for performing intravitreal injections in experimental mice．

Key words: intravitreal injection, glass capillary, microglia