2型糖尿病肾病患者肾组织中STOML2的表达及作用

doi:10.3969/j.issn.1000-8535.2016.04.013

广州医药 ›› 2016, Vol. 47 ›› Issue (4): 42-44.DOI: 10.3969/j.issn.1000-8535.2016.04.013

2型糖尿病肾病患者肾组织中STOML2的表达及作用

陈浩雄, 傅君舟, 何凤, 刘日光

广州市第一人民医院肾内科(广州 510180)

收稿日期:2016-04-08 出版日期:2016-07-20 发布日期:2021-12-02
通讯作者: 傅君舟,E-mail:fujzhou@163.com
基金资助:
广州市医药卫生科技引导项目(20151A010019)

The role of STOML2 in renal tissue of patients with type 2 diabetic nephropathy

Chen Haoxiong, Fu Junzhou, He Feng, et al

Department of Nephrology, Guangzhou First People's Hospital, Guangzhou 510180,China

Received:2016-04-08 Online:2016-07-20 Published:2021-12-02

摘要/Abstract

摘要： 目的探讨2型糖尿病肾病(DN)患者肾组织中STOML2的表达及作用。方法免疫组化检测临床2型糖尿病肾病患者肾组织STOML2的表达及定位,采用慢病毒转染方法建立稳定过表达STOML2的HK-2细胞系,并应用Western blot检测肾小管上皮细胞钙粘蛋白(E-cadherin)、Fibronectin和STOML2的表达。结果 STOML2在DN患者肾组织的肾小管上皮细胞胞浆中表达明显升高。在高糖刺激HK-2细胞建立的EMT模型中,STOML2呈时间依赖表达上调。STOML2稳定高表达时,E-cadherin表达下调,而Fibronectin明显上调,即能促进肾小管上皮细胞发生EMT。结论 STOML2可能通过促进肾小管上皮细胞向间充质细胞分化,进而参与糖尿病肾病肾脏纤维化的发生发展。

关键词: 糖尿病肾病, 上皮细胞-间质转化, 纤维化, STOML2

Abstract: Objective To investigate the expression and role of STOML2 in renal tissue of patients with type 2 diabetic nephropathy. Methods To detect he expression and localization of STOML2 in clinical renal tissue in patients with type 2 diabetic nephropathy by immunohistochemistry, and use lentiviral transfection method to establish a stable cell line of over-expressing STOML2, lastly apply western blot to detect the expression of E-cadherin, Fibronectin and STOML2 in renal tubular epithelial cells. Results STOML2 was significantly increased in renal tubular epithelial cytoplasm of patients with DN. In the EMT model of HK-2 cells stimulated by high glucose, STOML2 was increased in a time dependent. Overexpression of STOML2 led to E-cadherin down-regulated, while Fibronectin up-regulated, which promoted the occurrence of EMT in renal tubular epithelial cells. Conclusion STOML2 may be involved in the development and progression of renal fibrosis in diabetic nephropathy by mediating epithelial- mesenchymal transition of renal tubular epithelial cells.

Key words: Diabetic nephropathy, Epithelial-mesenchymal transition, Fibrosis, STOML2

陈浩雄, 傅君舟, 何凤, 刘日光. 2型糖尿病肾病患者肾组织中STOML2的表达及作用[J]. 广州医药, 2016, 47(4): 42-44.

Chen Haoxiong, Fu Junzhou, He Feng, et al. The role of STOML2 in renal tissue of patients with type 2 diabetic nephropathy[J]. Guangzhou Medical Journal, 2016, 47(4): 42-44.

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2型糖尿病肾病患者肾组织中STOML2的表达及作用

The role of STOML2 in renal tissue of patients with type 2 diabetic nephropathy

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