关键词: 非编码长链 RNA ANRIL, miR-181b, 磷酸酶及张力蛋白同源物基因, 冠心病, 心肌损伤

Abstract: Objective To explore the mechanism of competitive binding of non coding long stranded RNA ANRIL（lncRNA-ANRIL）to miR-181b to mediate phosphatase and tensin homolog gene（PTEN）on myocardial injury in coronary heart disease．Methods Thirty patients diagnosed with coronary heart disease in our hospital from October 2023 to June 2024 were included as the observation group,and another 30 individuals from physical examination center during the same period were selected as the control group．Blood pressure indicators,blood lipid indicators,and serum levels of lncRNA-ANRIL,miR-181b,and PTEN were measured in the two groups of patients,and the test results were compared．Results There was no significant difference between the two groups in terms of gender,age,BMI,smoking and hypertension（P>0.05）．The levels of systolic blood pressure（SBP）,diastolic blood pressure（DBP）,total cholesterol（TC）,triglycerides（TG）,and low-density lipoprotein cholesterol（LDL-C） in the observation group were higher than those in the control group,while high-density lipoprotein cholesterol（HDL-C） was lower than that in the control group（P<0.05）．The relative expression levels of lncRNA-ANRIL Exon 1-2,Exon 17-18,and PTEN levels in the observation group were lower than those in the control group（t=12.623,7.741,8.231,P=0.001）,while the level of miR-181b was higher than that in the control group（t=37.250,P=0.001）．Conclusions Compared with healthy individuals,serum levels of lncRNA-ANRIL and PTEN are significantly reduced in patients with coronary heart disease,while miR-181b levels are elevated,indicating that lncRNA ANRIL can regulate PTEN expression by miR-181b,thereby affecting the process of myocardial injury in coronary heart disease．

Key words: lncRNA-ANRIL, miR-181b, PTEN, coronary heart disease, myocardial damage